Industrial Wastewater Bacteria, Resistant to lead, Selenium and Antibiotics

Document Type : High quality original papers

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Botany and Microbiology Department, Faculty of Science, Sohag University 82524, Sohag, Egypt.

Abstract

Environmental pollution with heavy metals is a serious problem facing most countries. This problem is correlated always with misuse of different industrial products and wrong treatment of industrial waste. Presence and prevalence of heavy metal resistant bacteria consider an indicator of contamination with heavy metals. This work was performed to support the evidence of presence of heavy metal resistant bacteria in polluted environment, and to examine the changes occur in bacterial morphology under the stress of heavy metal ions occurrence. Water samples were collected from industrial waste, Riyadh Saudi Arabia. Bacterial isolates were identified using VITEK System version: 04.01. The antibiotic resistance of bacterial isolates were also examined using the standardized single disk method. The bacterial morphology was examined using scanning electron microscope to clearify whether the bacterial cell characteristics are changed. The MICs (Minimal Inhibition Concentration) were determined in triplicate by growing the bacterial strains on NA plates containing different concentrations of lead and Selenium as well. Three bacterial isolates were identified as Escherichia coli, Klebsiella pneumonia, Bacillus sp. The strain of Klebsiella pneumoniae was resistant to 15% of used antibiotics, Bacillus sp. isolate was resistant to 30%, while Escherichia coli, was resistant to 40% of tested antibiotics. The higher lead MIC value was detected with Klebsiella pneumonia (1100 ppm). The cell sizes of the isolated bacteria were slightly changed according to scanning electron microscopy. Growing of E. coil on nutrient agar amended with selenium oxide resulted in selenium particles size around 270 nm.

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