Glucophilic fungi of sugarcane bagasse in Egypt

Document Type : High quality original papers

Authors

1 Botany and Microbiology Department, Faculty of Science, Sohag University 82524, Sohag, Egypt.

2 Microbiology Department, Soil, Water & Environment Research Institute, Agricultural Research Center, Giza, Egypt.

3 Microbiology Department, Soil, Water & Environment Research Institute, Agricultural Research Center, Shandaweel Research Station, Sohag, Egypt.

Abstract

Thirty sugarcane bagasse samples were collected from different sugarcane juice mills in Sohag Governorate, Upper Egypt. The moisture content of tested samples was fluctuated between 32.58 - 70.59%. Total carbohydrates were estimated, and the richest sample was No. 21 with 535.10 mg/g dry weight, whereas the lowest sample was No. 3 with 508.131 mg/g dry weight. Soluble sugars of different samples were determined using spectrophotometer at 620 nm. The soluble sugars content ranged between 120 - 160 mg/g dry weight. Dilution- (8 genera, 27 species and 4 varieties) and direct- (8 genera, 25 species and 4 varieties) plate methods on glucose-Czapek's agar medium at 28 ±2 oC were used for isolation of bagasse glucophilic fungi. It was found that the total fungal populations in case of dilution–plate method (69720 cfu/g) was so high than those of direct–plate method (828 cfu/ 20 bagasse segments). Aspergillus was appeared in high population and was parallel to those of total fungal counts (51700 cfu/g, 74.15 % of total count, in 29 samples with 96.7% of the samples in dilution method and 731 cfu/ 20 segments, 88.3% of total count, in 30 samples with 100% of the tested samples using direct-plate method.

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