Screening and optimization of extracellular cellulase and pectinase enzymes produced from post-harvest fungi of apple (Pyrus malus L.) and tomato (Solanum lycopersicum L.)

Document Type : High quality original papers

Authors

1 Biology Department, Faculty of Science, Taif University, Kingdom of Saudi Arabia.

2 Botany Department, Faculty of Science, South Valley University, 83523 Qena, Egypt

3 Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt.

Abstract

Fourty-eight species and two species varieties belonging to 19 genera were collected from 50 samples of apple and tomato fruits (25 from each) from Sohag governorate in Egypt on dichloran rose-Benegal chloramphenicol agar medium at 28ᴼC. The most common genera were Alternaria, Aspergillus, Cladosporium, Fusarium and Penicillium. Form the above genera the most prevalent species were Alternaria alternata, A. tenuissima, Aspergillus niger, A. flavus, A. fumigatus, Cladosporium cladosporioides, C. herbarum and Penicillium oxalicum. Fourty-eight species and two species varieties fungal species screened for their abilities to produce cellulase (C1 enzyme) and pectinase enzymes. Six and seven species showed high cellulolytic and pectinolytic activity, while 23 and 17 species were moderately activity for the two enzymes, respectively. The remaining species were low activity in both enzymes. The highest cellulase and pectinase activities were recorded by Aspergillus chevalieri. Maximum production of cellulase enzyme by A.chevalieri was obtained after 6 days of incubation at 30ᴼC with initial pH 6 in culture medium containing sucrose and peptone as carbon and nitrogen sources, respectively. Regarding to pectinase enzyme the highest pectinase production by A. chevalieri was recorded after 6 days of incubation at 30ᴼC with initial pH 8 in culture medium containing pectin and ammonium sulphate as carbon and nitrogen sources, respectively.

Keywords

Main Subjects

Files

Share

How to cite

Statistics