Chromatographic separation and identification of many amino acid compounds of Leucaena glauca L. growing in Iraq

Al-Daoody, Ayad; AL-Hashemi, Fanar H.; Shehab, Ahlam A.

doi:10.21608/jesj.2015.197498

Chromatographic separation and identification of many amino acid compounds of Leucaena glauca L. growing in Iraq

Document Type : High quality original papers

Authors

¹ Department of Biology, College of Education, University of Mosul, Iraq.

² Department of Horticulture and Landscape Design, College of Agriculture and Forestry, University of Mosul, Iraq.

10.21608/jesj.2015.197498

Abstract

The aim of the present study was to separation and investigation of many amino acids (glycine, aspargen, leucine, glutamic acid, proline, argenine, valine, alanine) from the leaves and seeds of leucaena glauca L. Amino acids were separated and investigated by highperformance liquid chromatography (HPLC) technique, which consisted of a UV-vis detector, C18, Mobile phase was Na2SO4. The result showed that the sample of chloroform extract of leaves was recorded of the highest quantity of aspargine (43.725%), leucine (8.905%) glutamic acid (4.300%), while leucine, proline, alanine were recorded the highest quantity in seeds (29.575%, 19.031%, 14.115%) respectively.

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