Apyrase like proteins and epigenetic roles during initial germination of (Pisum sativum L.) seedlings

Moustafa, Mahmoud; Hafez, Elsayed E.

doi:10.21608/jesj.2010.184378

Apyrase like proteins and epigenetic roles during initial germination of (Pisum sativum L.) seedlings

Document Type : High quality original papers

Authors

Mahmoud Moustafa ¹
Elsayed E. Hafez ²

¹ Botany Department, Faculty of Science, South Valley University, Egypt.

² Mubarak City for scientific Research and Technology Applications, Alexandria, Egypt.

10.21608/jesj.2010.184378

Abstract

Apyrase is a CaM-binding proteins and it is playing an important roles in response to biotic and abiotic stresses during embryo development. The expression of different isotypes of 36 kDa and 46 kDa antigenic to apyrase in dry seeds, during imbibition, and initial germination were examined using 2D-gel and western blotting. Both of the two genes were highly expressed in dry seeds and imbibiation ones but they were completely disappeared during germination. Thirteen different isotypes for the 46 kDa was observed with pI 5.3, 5.4, 5.6, 5.8, 6.0, 6.3, 6.6, 6.8, 7.4, 7.5, 7.55, 7.65 and 7.7 while in case of the 36 kDa only two isotypes were demonstrated with pI 7.3 and 7.35. Further studies were carried out on both of the embryo protein content and ATPase activity, the protein content was decreased by increasing the ATPas activity during the embryo development. In conclusion, both of the 46 and 36 kDa proteins are two separate enzymes correlated to apyrase enzyme. In addition, the different isotypes of the both enzymes are kind of epigenetic effect which carried out by the highly developed cells to produce different forms of proteins. These proteins perform different function during the germination process. They suppress a default death pathway in plants.

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